1. Cloning vectors in yeast include yeast artificial chromosomes ( YACs ).2. They are often used as a cloning vector in genetic engineering. 3. One bacterial plasmid used in genetic engineering as a plasmid cloning vector is pUC18. 4. Genome size varies among different organisms and the cloning vector must be selected accordingly. 5. P1 was developed as a cloning vector by Nat Sternberg and colleagues in the 1990s. 6. A plasmid cloning vector is typically used to clone DNA fragments of up to 15 kbp. 7. This method requires that restriction maps of the cloning vector and the insert are already available. 8. His cloning vectors were also used to develop the method for oligonucleotide site-directed mutagenesis. 9. There are many types of cloning vectors , but the most commonly used ones are genetically engineered plasmids. 10. Formation of recombinant DNA requires a cloning vector , a DNA molecule that replicates within a living cell.
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